HOME > Outline of the Glycated Albumin (GA) > Reagent Features and Performance

Reagents for the Measurement of Glycated Albumin (GA) Lucica® GA-L

Reagent Features and Performance

Overview

Lucica® GA-L is supplied as a ready-to-use, liquid reagent kit, for the measurement of glycated albumin, which:

  • contains high-specificity protease and enzymes, and
  • can be utilized with general biochemical automated analyzers, and
  • delivers same-visit results prior to physician consultation, and
  • employs a novel BCP method that is highly specific to albumin.

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Assay Principle

Glycated albumin: Influence by endogenous glycated amino acids is avoided through the use of an elimination reaction.

Glycated albumin: Influence by endogenous glycated amino acids is avoided through the use of an elimination reaction.

Albumin: A novel BCP method permits more specific measurement of albumin.

Albumin: A novel BCP method permits more specific measurement of albumin.

Calculation of glycated albumin (GA) value

Calculation of glycated albumin (GA) value

Kouzuma T, et al. Clinical Chimica Acta 346:135-143 (2004)

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Assay Precision

Intra-day reproducibility (n = 20)
  Control Normal sera Diabetic sera
Mean 14.4% 15.9% 24.5%
SD 0.09 0.13 0.23
CV 0.63% 0.82% 0.93%

Inter-day reproducibility (n = 20)
  Normal sera Diabetic sera
Mean 16.2% 24.8%
SD 0.091 0.166
CV 0.56% 0.67%

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Reduced Frequency of Calibration

  Normal sera Diabetic sera
Mean 15.9% 24.3%
SD 0.109 0.182
CV 0.68% 0.75%

A CV of less than 1% was attained even though calibration was conducted only on the first day of a two-week period of assays.


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Interfering Substances

Bilirubin F interference
Bilirubin F interference

Chyle interference
Chyle interference

Ascorbic acid interference
Ascorbic acid interference
Bilirubin C interference
Bilirubin C interference

Glucose interference
Glucose interference

Hemoglobin interference
Hemoglobin interference

 

Bilirubin F and C, chyle, and glucose demonstrated almost no interference in the glycated albumin (GA) assay.

Ascorbic acid up to 100 mg/dL and hemoglobin up to 196 mg/dL demonstrated no interference in the GA assay.

Hemoglobin demonstrated slightly negative interference.


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Anti-coagulant/Glycolytic Inhibitor Interference

Anti-coagulant/Glycolytic Inhibitor Interference

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Linearity

Glycated albumin (g/dl)Albumin (g/dl)

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Correlation

Correlation

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Assay Examples

Glycated Albumin Reagents

Glycated Albumin Reagents


Albumin Reagents

Albumin Reagents
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Assessment of Glycemic Control

Glycated albumin (GA) values are about 3 times greater than glycated hemoglobin (HbA1c) values when glycemic control is stable.
  Excellent Good Acceptable Failure
GA < 18.0 18.1 to 21.0 21.1 to 24.0 24.1 <

Tahara Y. Glycoalbumin (GA). In: Shima K, editor.
Kettou Wo Miru Kangaeru.
Takyo: Nankodo; 2000. p62-69. (in Japan)

 

 

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