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Clinical Chemistry Enzymes and Reagents
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Reagents for the Measurement of Glycated Albumin (GA) Lucica™ GA-L
What is Glycated Albumin?
Is Glycated Albumin the Same as Fructosamine?
Characteristics of Glycated Albumin
Reagent Features and Performance
Reagents for the Measurement of MI
What is MI?
Features of MI
Reagent Features and Performance
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Enzyme for standarization
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Lucica(TM) GA-L for the measurement of glycated albumin

Reagent Features and Performance

Overview Assay Principle Assay Precision

Reduced Frequency of Calibration Interfering Substances

Anti-coagulant/Glycolytic Inhibitor Interference Linearity Correlation

Assay Examples Assessment of Glycemic Control

Overview

Lucica GA-L is supplied as a ready-to-use, liquid reagent kit, for the measurement of glycated albumin, which:
contains high-specificity protease and enzymes, and
can be utilized with general biochemical automated analyzers, and
delivers same-visit results prior to physician consultation, and
employs a novel BCP method that is highly specific to albumin.
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Assay Principle

Glycated albumin: Influence by endogenous glycated amino acids is avoided through the use of an elimination reaction.

Albumin: A novel BCP method permits more specific measurement of albumin.

Calculation of glycated albumin (GA) value

Kouzuma T, et al. Clinical Chimica Acta 346:135-143 (2004)
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Assay Precision

Intra-day reproducibility (n = 20) Inter-day reproducibility (n = 20)
Control Normal sera Diabetic sera
Mean 14.4% 15.9% 24.5%
SD 0.09 0.13 0.23
CV 0.63% 0.82% 0.93%
Normal sera Diabetic sera
Mean 16.2% 24.8%
SD 0.091 0.166
CV 0.56% 0.67%
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Reduced Frequency of Calibration

Normal sera Diabetic sera
Mean 15.9% 24.3%
SD 0.109 0.182
CV 0.68% 0.75%

A CV of less than 1% was attained even though calibration was conducted only on the first day of a two-week period of assays.
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Interfering Substances

Bilirubin F interference Bilirubin C interference
Chyle interference Glucose interference
Ascorbic acid interference Hemoglobin interference

Bilirubin F and C, chyle, and glucose demonstrated almost no interference in the glycated albumin (GA) assay.
Ascorbic acid up to 100 mg/dL and hemoglobin up to 196 mg/dL demonstrated no interference in the GA assay.
Hemoglobin demonstrated slightly negative interference.
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Anti-coagulant/Glycolytic Inhibitor Interference

Anti-coagulant/Glycolytic Inhibitor Interference
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Linearity

Glycated albumin Albumin
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Correlation

Correlation
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Assay Examples

Glycated Albumin Reagents

Albumin Reagents
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Assessment of Glycemic Control

Glycated albumin (GA) values are about 3 times of glycated hemoglobin (HbA1c) values when glycemic control is stable.
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Asahi Kasei Pharma Corporation. All rights reserved.