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Lucica MI is an in vitro diagnostic test kit for the measurement of UMI before and after glucose loading. |
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Measurement of UMI requires a urine sample, but no blood sample is needed. This test may thus be suitable for mass screening for both diabetes and glucose intolerance. |
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Lucica MI is a highly sensitive test kit based on an enzymatic method that uses liquid reagents requiring no preparation. |
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Lucica MI can be used in general-purpose biochemical autoanalyzers. |
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| Myoinosose is formed from MI by reaction with myoinositol dehydrogenase (MIDH). At the same time, thio-NADH is formed from the coenzyme thio-NAD. Through the same reaction with MIDH, using NADH as a coenzyme, MI is formed from myoinosose. This is used again in the prior reaction (enzyme cycling). The rate of increase in thio-NADH, dependent on MI concentration, is measured by absorbance changes at a wavelength of 405 nm. This quantifies the concentration of MI. |
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 Within-run |
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Between-run |
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Urine-1 |
Urine-2 |
Urine-3 |
Mean
(μM) |
104.2 |
351.9 |
1086.8 |
| CV(%) |
1.1 |
0.7 |
0.5 |
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Urine-1 |
Urine-2 |
Urine-3 |
Mean
(μM) |
105.8 |
352.1 |
1084.9 |
| CV(%) |
1.3 |
0.4 |
0.6 |
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| Substrate |
Relative activity % |
| Myoinositol |
100 |
| D-chiroinositol |
18 |
| Glucose |
0 |
| Sorbitol |
0 |
| Fructose |
0 |
| 1,5Anhydroglucitol |
0 |
| Galactose |
0 |
| Mannose |
0 |
| Mannitol |
0 |
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| Substance |
Concentration range with no effect on assay value |
| Ascorbic acid |
 500mg/dL |
| Oxalic acid |
500mg/dL |
| Tartalic acid |
500mg/dL |
| Sodium tricitrate |
500mg/dL |
| Ammonium chloride |
500mg/dL |
| Creatinine |
500mg/dL |
| Urea |
500mg/dL |
| Galactose |
50mg/dL |
| Uric acid |
50mg/dL |
| Glucose |
2g/dL |
| Hemolyzed hemoglobin |
96.8mg/dL |
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| Glucose at concentrations > 2g/dL can cause false-negative errors. Creatinine at concentrations exceeding 500mg/dL can cause false-positive errors. |
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| This figure shows the dilution linearity of 4 different urine samples. In all case, good linearity was shown. |
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