Enzyme Product List List All Dehydrogenase Hydrolase Isomerase Ligase Lyase Oxidase Transferase   Enzyme for standarization   Multi-Enzyme (Human) Calibrator With the multiplicity of measurement methods and conditions employed in the field of clinical chemistry, measurement values of the same sample differ between laboratories. The elimination of interlaboratory differences is sought through the standardization of test results. Among routine diagnostic tests, variations are more prominent with enzymatic tests in particular than with electrolytic tests. To eliminate differences seen with enzymatic testing, the promotion of Recommended Methods to be used as Reference Methods and the distribution of Reference Materials as reference points are necessary. The Japan Society for Clinical Chemistry has promoted its Consensus Methods for six types of enzymes (AST, ALT, gamma-GT, ALP, CK, LD). Since 1997 the Japan Association of Clinical Reagent Industries has distributed the Japan Working Enzyme Reference Material with recombinant enzymes. Through these efforts, the interlaboratory differences in enzymatic testing have been largely corrected in Japan. Asahi Kasei Corporation produces a lyophilized preparation with recombinant enzymes in a BSA matrix as an enzyme calibrator, which occupies a position immediately below Reference Materials. After further development in the areas of handling and safety, Asahi Kasei now introduces its latest recombinant enzyme calibrator as described below. Lyophilized form composed of multipleenzymes along with BSA Multi-Enzyme Calibrator Origin of Enzymes 8 kinds (AST, ALT, gamma-GT, ALP, CK, LD I, p-AMY, Lipase) recombinant E. coli recombinant mammalian cell Matrix bovine serum albumin (BSA) ---non-biohazard Form lyophilized Packaging 3 ml/vial (tentative) Reactivity similar to human serum Physicochemical properties 1) specific gravity 2) viscosity similar to human serum similar to human serum Stability more than 18 months at 5°C 8 hours at 5°C after reconstitution 1 Human gene recombinant AST, ALT, gamma-GT, ALP, CK, LD (isozyme I type), p-AMY, p-Lipase Data - Isozyme Type and Source 2 Minimal loss of activity after reconstitution Data - Activity following Reconstitution 3 Excellent calibration effect Data - Example of Calibration Effect 4 Similar reactivity as human sera Data - Recombinant Enzyme Correlation with Human Sera 5 Homogeneity Data - Vial-to-Vial Difference of Each Enzyme Among 20 Vials 6 Long-term Stability Data - Stability at 5°C Enzyme Isozyme type Source Lyophilized Lyophilized Type A Type B AST Cytosolic recombinant E. coli + + ALT Cytosolic recombinant E. coli + + gamma-GT II (liver) recombinant mammalian cell + + ALP liver recombinant mammalian cell + + CK MM recombinant E. coli + + LD I, II, III human red blood cell + - I recombinant E. coli - + P,S-AMY Pancreatic, saliva human urine, human saliva - - P,S-AMY Pancreatic, saliva recombinant yeast, human saliva + - P-AMY Pancreatic recombinant yeast - + Lipase Pancreatic recombinant mammalian cell + + [Return to Characteristics]   [Return to Characteristics] [Return to Characteristics]   [Return to Characteristics] N = 20 AST ALT gamma-GT ALP CK LD AMY CV (%) 0.69 0.93 0.78 0.59 0.71 0.77 0.62 [Return to Characteristics] [Return to Characteristics]

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