Summary of the Test Item

• Levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) are test markers for disorders such as dyslipidemia.
• Cholesterol levels are assayed using the enzymatic method.

Asahi Kasei Solutions

• We supply cholesterol esterase and cholesterol oxidase for the cholesterol levels measurement. Our products include two types of cholesterol esterase (CENⅡ and CEBPⅡ) with different properties, which may be used as appropriate depending on the target of the assay (TC, HDL-C, or LDL-C) and/or the formulation.

• With a highly hydrophobic enzyme such as cholesterol esterase, it is important to select appropriate addtives.
We have data on the surfactants and salts that increase the activities and stabilities of CENⅡ and CEBPⅡ, which may facilitate the acceleration of your product development.
Please contact us for details.

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Measurement Principle
Performance Comparison Chart of Asahi Kasei Enzymes

Cholesterol Esterase

Cholesterol Oxidase



CONⅡ-FD (T-101)


High activity,
High stability

Low adsorption

Low Km value

Optimum pH




pH stability

pH stability

Stable in low pH


Thermal stability

Stable at <45℃

Stable at <45℃

Stable at <65℃

Related Information

Press Releases

CEN II press release:

Product Information

Please contact us if you are looking for other products not listed below.
Asahi Kasei Pharma Diagnostics Division carries many solutions and products.
We may be able to meet your needs.

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